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Whitley H45 HEPA Hypoxystation

The H45 HEPA Hypoxystation is a large, 3-port cell culture workstation ideal for those requiring HEPA filtration, as well as being able to accurately control oxygen, carbon dioxide, temperature and humidity. Ensure your samples are protected in the best, most reliable environment possible.

  • Control O2 in 0.1% increments up to 20%; CO2 in 0.1% increments up to 15%; and relative humidity up to 80% - provides flexibility in your research and confidence in your results.
  • Fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter every four seconds, which ensures the chamber environment is cleaned quickly. Placing the filters inside the workstation prevents the filter becoming saturated with moisture.
  • Rather than use off-the-shelf filters, DWS has worked with one of the world's leading filter manufacturers to develop a filter specifically tailored to suit this Hypoxystation.
  • Levels of atmospheric cleanliness inside the workstation exceeds the requirements of ISO 14644 Class 3 (equivalent to Class 1 of the US Fed Std 209E Cleanroom Standards and exeeds the particle counts attained at rest’ (without an operator) of Grade A as set out in the GMP Guidelines). We can provide test results confirming particulate levels inside the workstation.
  • Fitted with a fan that circulates the internal atmosphere continuously - contributing to even temperature distribution and ensuring any internal particulate will be in motion.
  • Has a removable front as standard to facilitate the transfer of bulk samples or larger pieces of equipment for use in the workstation. No special tools are required and there are no loose parts to store.
  • 50% larger than a H35 Hypoxystation, the additional space inside the H45 allows you to marshall your experiments more efficiently and study your samples using various pieces of equipment inside the workstation environment.
  • With three entry ports, one instant access port for transferring and retrieving samples quickly (and efficiently – no need to run an airlock cycle) and two oval sleeved ports for comfortable working inside the cabinet.
  • Built-in rapid airlock to ensure samples can be transferred into the workstation atmosphere as soon as possible (only takes 60 seconds).
  • Colour, touch-screen control panel for ease of use and for visual display of parameters such as O2 level, CO2 level, temperature, humidity, and airlock cycle status.
  • Compact footprint with fully integrated gas control – no bulky add-on systems to find  additional bench space for.
  • CO2 Monitoring device fitted as standard to enable you to experiment using different concentrations of CO2.
  • Optional automatic humidification system provides a sterile source of humidity to prevent media evaporation during extended incubation.
  • Two point oxygen sensor calibration that takes only 6 minutes. Easy calibration using the touchscreen control panel and you don’t have to remove the oxygen sensor from the workstation and risk a build-up of condensation, which could in turn lead to inaccurate readings. 
View Specifications


Capacity (Litres) 450
Port / Airlock Capacity Accommodates up to 44 x 96 well plates or 84 x T25 culture flasks
Airlock Size 12 litres / 223 x 190mm (d x h)
Porthole System 1 x Instant Access Port + 2 Manual Oval Sleeved Ports
Gas Supplies CO2 / Air/ N2
Footswitch Wireless
Auto Sleeve Gassing Option available
Internal Mains Socket Option available
Storage Trays Option available
Inspection Lamp Option available
Single Sample Entry Option available
Data Logging Option available
Airlock Cycle Time 60 Seconds
Extra Cable Glands Option available
Microscope Option available / Please contact us for more information
Automatic Dehumidifier Fitted as standard
Automatic Humidifier Option available
Removable Front Fitted as Standard
Trolley Option Available
Dimensions (w/d/h - mm) 1660 x 810 x 710
Weight (lbs/kg) 386 / 175
CO2 Monitoring Fitted as standard
Vacuum Take-off Option available
Temperature Range 5°C above ambient to 45°C
Touchscreen Control Yes
HEPA Fitted as standard


All accesssories can be purchased at the Don Whitley Scientific main site.

A02945  Spare Cable Gland

A06102  Vacuum Take-Off

A06103  9cm Front Loading Letterbox (Single sample entry)

A06104  Whitley Automatic Humidification System

A06106  Double Internal Socket

A06107  Gas Sample Port

A06128  Large Additional Cable Gland

A06114  Internal Storage Tray

A06116  Automatic Sleeve Gassing System

A06127  Short Internal Shelf

A06655  Replacement HEPA Filter (device requires x2)

A07202  H45 Workstation Trolley

A07208  9cm Letterbox (Single sample entry)

A07211  Workstation Spotlight

A07218 Whitley Enhanced Biological Containment System

L00040  Temperature Mapping

L00050  Single Point Temperature Calibration


Clicking the links below will take you to the consumables on the Don Whitley Scientific main site.

A01923 Foam Cleanser (UK Only)
A02672 Sleeve Retaining O'Ring
A02709 Footswitch Battery - AAA
A02715 Workstation PVC Sleeve
A02716 Plastic Sleeve Cuff Ring
A02820 Sleeve Kit with Latex Wrist Seal
A02821 Latex Wrist Seals
A02830 Sleeve Kit with Neoprene Wrist Seal (Standard)
A02831 Neoprene Wrist Seal (Standard)
A02840 Sleeve Kit with Neoprene Wrist Seal (Large)
A02841 Neoprene Wrist Seal (Large)
A02535 Tray Pushrod
A03780 Storage Rack
A06928 Storage Rack with Trays
A03912 Whitley Gas Bottle Holder
A00202 Neoprene Gauntlets
A00118 Latex Gauntlets