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Whitley H135 HEPA Hypoxystation

The H135 HEPA is the tallest, widest, deepest hypoxic chamber in the Whitley range. It has a usable internal volume of over 550 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc. The generous internal height facilitates easy pipetting. All cell manipulations can be performed without removing them from your required hypoxic conditions. The entire cell culture incubator chamber is a working and incubation space.

This workstation provides precise environmental control whilst leaving plenty of room to work, incubate and conduct analysis.

  • Precise control of O2, CO2 and N2 – provides flexibility in your research and confidence in your results.
  • The Whitley Internal HEPA Filtration System with Enhanced Biological Containment.
  • A removable front (fitted with two patented oval ports) to facilitate the transfer of large items of equipment for use inside the chamber.
  • Innovative full colour touch screen that is Ethernet-enabled for remote access. Now you can log into your workstation when you are away from the lab.
  • A rapid 12 litre airlock – no risk of compromising conditions inside the chamber (and it’s flushed with nitrogen as an additional cost saving).
  • Fully automated O2 calibration routine (patent pending) – activated via the touchscreen with no need to touch or remove the sensor - saves time, no risk of user error and ensures the accuracy of your results.

Options Include:

  • The Whitley Automatic Humidifier – sterile, on-demand moisture addition.
  • For those who want to use a Seahorse XF Analyzer in their work, there is the option of connecting the H135 HEPA to a Whitley i2 Instrument Workstation.



Specification liable to change without notice

View Specifications


Capacity (Litres) 826
Port / Airlock Capacity 12 litres / Accommodates up to 44 x 96 well plates or 84 x T25 tissue culture flasks
Airlock Size 223mm Deep x 190mm High
Porthole System Manual, oval ports
Gas Supplies CO2, N2, Air
Footswitch Wireless
Auto Sleeve Gassing Option Available
Internal Mains Socket Option Available
Storage Trays Option Available
Inspection Lamp Option Available
Single Sample Entry Option Available
Data Logging Option Available
Airlock Cycle Time 60 Seconds
Extra Cable Glands Option Available
Automatic Dehumidifier Fitted as Standard
Automatic Humidifier Option Available
Removable Front Fitted as Standard
Trolley Not Available
Dimensions (w/d/h - mm) 1452/1056/993
Weight (lbs/kg) 386/175
CO2 Monitoring Fitted as Standard
Vacuum Take-off Option Available
Touchscreen Control Yes
HEPA Fitted as Standard